The two most cited routes for injecting clodronate liposomes into the CSF is ... Encapsula NanoSciences LLC 5409 Maryland Way Suite 360 Brentwood, TN 37027 Phone: 1-800-387-0620 Phone: 1-800-401-5140 Phone: 1-800-410-0640 Phone: 615-884-4442 Fax: 615-250-8747 URL: www.encapsula.com email: info@encapsula.com Disclaimer. formulations sold on the website are for research purposes only. Adminstration by Retro-orbital Injection, Publications Reporting Monocyte Depletion by I.V. Intravenous dosing has also been shown to deplete over 60% of alveolar macrophages in mice 48 hours post-treatment, while a combination of intracheal injection and i.v. Tissues were collected, frozen, and stored at −80 °C until use. Mice were sacrificed after 0h or 6h or 24h post-reperfusion, and liver and serum samples were collected. In osmotically active liposomes the lipid membrane is permeable and the … Rat anti–IFN-γ monoclonal antibody (clone: XMG1.2, BioXcell), clodronate and empty liposomes were from Encapsula NanoSciences (Brentwood, TN). Clodronate‐containing liposomes have been widely used experimentally for the selective depletion of circulating monocytes . Suite 360 dosing resulted in over 90% depletion. Recipient SR‐AI –/– mice were treated with 100 μL of clodronate liposome suspension (Encapsula NanoSciences, Brentwood, TN) by tail vein injection 1 day preceding transfer of 2 × 10 6 differentiated bone‐marrow–derived macrophages (BMDMs), also by tail vein injection. 15. Liposome Basics: Part I. Liposome Basics: Part II. Read More. or ID) of clodronate liposomes has been used for elucidating the immune mechanisms involved with skin diseases, such as psoriasis, dermal infections (including systemic infections obtained through the skin) and other antigenic reactions in the skin. Osmotic pump (Alzet model 1007D, 100 ml; 0.5 ml/h), cannula (Azlet brain infusion kit 3) were from Charles River, Italy. Phone: 1-800-410-0640 Liposomal clodronate (Encapsula NanoSciences, Brentwood, TN) was injected (200 μL, i.p., twice a week) followed by anti‐PD1 treatment 24 h later. Classic. Fax: 615-250-8747 The code will save the url of this webpage to the device for mobile sharing and storage. If the encapsulated molecule is temperature sensitive (e.g. Liposome: Encapsula's Scientific Blog. We offer one year shelf-life guarantee from the time of delivery to the date of reconstitution. Liposome made from pure phospholipids (in the absence of cholesterol) will not form at temperatures below the phase transition temperature of phospholipid. For monocytic or granulocytic cells depletion, animals received single vs. continuous (twice per week) intraperitoneal (i.p.) Classic; Flipcard; Magazine; Mosaic; Sidebar; … Single dose liposomal clodronate (200 μL/animal i.p., Encapsula NanoSciences LLC, Brentwood, TN) was used to deplete Kupffer cells. Macrophage Depletion Reagents (Clodrosome®), Liposomal Doxorubicin (Doxosome™ and Immunodox®), Liposomes for DNA/RNA Delivery (Genesome®), Lyophilized Liposomes for Formulators (Lyophosome™), Fluorescent Control Liposomes for Tracking, Sulfhydryl Reactive Doxorubicin Loposomes. Depletion efficiency was determined by microscope examination of CX 3CR1 GFP cell density in muscle tissue. Learn the benefits and appropriate techniques of administering the macrophage depletion kits. Phone: 615-884-4442 Western blotting Lung nodules were homogenized, lysed using RIPA (Santa Cruz, TX), and 10% SDS/PAGE was run … The entry of nanoparticles into solid tumours . There are five fluorescent control liposome products (Fluoroliposome®) for Clodrosome® (clodronate liposomes). Dichloromethylene diphosphonic acid (clodronate)‐loaded liposomes (Encapsula NanoSciences, Brentwood, TN, USA) were injected i.v. a protein) then pure long chain saturated lipids can not be used in the liposomes because the lipids have to be heated up and the protein can not tolerate high temperatures. Aortic ring … The study with clodronate liposomes (CL) or PBS liposomes (PBSL) (Encapsula NanoSciences, Brentwood, TN, USA) was initiated in 4 week old mice and terminated when the mice reached the age of 8 weeks. Learn the basics of liposomology, macrophage depletion technique, the mechanism of action of clodrosome, and proper administration techniques. (Clodrosome; Encapsula NanoSciences Brentwood, TN, USA) 24 h before transplantation, and then, 50 µl intrascrotally every 48 h thereafter. Classic; Flipcard; Magazine; Mosaic; Sidebar; Snapshot; Timeslide ; Jan. 24. Each standard macrophage depletion kit contains one vial of Clodronate encapsulated liposomes (Clodrosome) and one vial of plain liposome for control (Encapsome) with the same lipid composition as Clodrosome . Injection, Publications Reporting Spleen Macrophage Depletion by I.V. Currently, a single paper cited intrarectal administration of clodronate liposomes [1]. The Macrophage Depletion Kits each contain one vial of a clodronate liposome suspension and one vial of a control liposome suspension. 5409 Maryland Way Brentwood, TN 37027 Isolation and culture of hepatic macrophages Colon and liver were diced into 5 mm pieces, and 25–30 of these pieces were placed in a 50-ml conical containing 25 ml RPMI 1640, supplemented with 3% FCS (R3). The content of the website can only be used by researchers, educators and students for The slow injection rate allows the liposome suspension to be thoroughly mixed into the blood so that they are properly opsonized for optimal phagocyte uptake. Any commercial use of the content of the website is legally prohibited Use this unique QR (Quick Response) code with your smart device. Both types of Clophsome ® are offered in the standard suspension form fo rthe convenience of use, the high potency form fo rdosing of high cotent with limited small volume and the lyophilized forms for the long shelf-life. % Control Cells Remaining Post-Clodronate Liposome Treatment [custom_table] Tissue 16 h 24 h 48 h; Bone Marrow : 40: 75: 130: Blood Monocytes: 5: 10: 110: Spleen: 1: 1: 1: Numbers extrapolated from supplemental figure 1 in paper. The animals should be closely observed for a few minutes for adverse reactions after the injection is completed and the animal returned to the cage. Search for: Home; The Company. Clodrosome® Liposomal Clodronate Suspension - 5 ml in glass vial Contains: Clodronate ((dichloro-phosphono-methyl)phosphonate) Disodium Salt 5 mg/ml L-α-Phosphatidylcholine 18.8 mg/ml Cholesterol 4.2 mg/ml Suspended in Phosphate Buffered Saline at pH 7.4 2. If those who will be working directly with the Clodrosome products are not experienced in handling and dosing liposomes, you may want to order extra reagents and animals. by the laws of the United States of America. By simple reconstitution in water one gets simply the best clodronate liposome products on the market. Explore the material safety data sheets (MSDS) for each available macrophage depletion kit product. After the removal of the organic solvent in a rotary evaporator, the large unilamellar liposomes of about 1 micrometer are formed. Moreover, clodronate pre-treatment increased durable donor-specific BALB/c skin allograft tolerance. Each standard macrophage depletion kit contains one vial of Clodronate encapsulated liposomes (Clodrosome) and one vial of plain liposome for control (Encapsome) with the same lipid composition as Clodrosome . Clodrosome (150 l/mouse; Encapsula NanoSciences, Brentwood, TN, USA) or encapsome (150 µl/mouse, serves as control; Encapsula NanoSciences) 19 was injected i.t. Page 1 of 1 1. Control mice were injected with 200 to 300 μL of … All animal care and procedures were carried out according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” prepared by the National Academy of Sciences and published by the National Institutes of Health (NIH publication … Macrophage Depletion Kit (Mannosylated) Each mannosylated macrophage depletion kit contains one vial of Clodronate encapsulated mannosylated liposomes (m-Clodrosome) … (EMD Millipore, Billerica, MA); Macrophage Depletion Kit (Encapsula NanoSciences LLC, Brentwood, TN) was used to deplete KCs according to manufacturer’s protocols. Each standard macrophage depletion kit contains one vial of Clodronate encapsulated liposomes (Clodrosome) and one vial of plain liposome for control (Encapsome) with the same lipid composition as Clodrosome, Each mannosylated macrophage depletion kit contains one vial of Clodronate encapsulated mannosylated liposomes (m-Clodrosome) and one vial of plain mannosylated liposome for control (m-Encapsome). Few details of the the instillation method were provided and it is unclear as to whether a polycarbonate tip on an automatic pipettor or a glass-capillary type micropipette was used for the instillation of the liposome suspension. [/custom_table] MΦ/monocytes or neutrophil depletion reduces the allogenic non-self response (pinnae swelling or DTH) by 50%. HFD‐fed mice received intraperitoneal (i.p.) into mice (200 μL/mouse), as previously described. Encapsome® is formulated and prepared identically to Clodrosome® except that clodronate is not added to the liposomes. CD4+ T cells, but not non-classical monocytes, are dispensable for the development of chronic lymphocytic leukemia in the TCL1-tg murine model A new publication in Nature Materials by a group in the University of Toronto in Canada. Read More. Clodrosome®, Encapsome®, Doxosome®, Immunosome®, Genesome®, Fluoroliposome®, ATPsome®, Cellsome®, Immunodox® and … Prepared identically to Clodrosome (without clodronate; with fluorophore) Required control since phagocytes can respond to liposome uptake; May be tracked by fluorescence methods (confocal microscopy, FACS, etc.) Interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) have been implicated historically in the immune pathophysiology of aplastic anemia (AA) and o… Mice were retro-orbitally injected with 200 or 300 μL of a clodronate-loaded liposome suspension (Encapsula NanoSciences, Brentwood, TN) every other day for four doses during the week prior to BM transplantation and beginning immunosuppression. Encapsula NanoSciences LLC 5409 Maryland Way Suite 360 Brentwood, TN 37027 Phone: 1-800-387-0620 Phone: 1-800-401-5140 Phone: 1-800-410-0640 Phone: 615-884-4442 Fax: 615-250-8747 URL: www.encapsula.com email: info@encapsula.com All five fluorescent liposomes incorporate a lipophilic dye inside their membranes. educational purposes. 7-Cl-O-Nec-1 (Nec-1s, 4mg/kg) or zDEVD-fmk (3.3mg/kg) was administered 1 hour prior to the ischemia, i.p. Mice were sacrificed on the third day of L635 administration. To learn more about the dosage for depleting various populations of macrophages, using various routes of administration, and different animal models visit the database. The good news is now we are offering lyophilized Clophosome® with a guaranteed 12 month shelf-life. MSDS Encapsome CL_Clodronate Liposome Control-Encapsula NanoSciences_2012 1. Control mice were given empty liposomes (Encapsome; Encapsula NanoSciences) following the same protocol. Liposomal clodronate (Clodrosome) and control liposome were pur-chased from Encapsula NanoSciences (Brentwood, TN, USA). Currently, a single paper cited intrarectal administration of clodronate liposomes [1]. Clodronate molecules are encapsulated in small MLV liposomes for superior activity, physical and chemical stability, and convenience of use and handling. View more documents from Encapsula NanoSciences For more info go to www.clodrosome.com. Just reconstitute by adding sterile pure water and mix (see more details below and the product sheet). Thus, all macrophage depletion studies should take into account the potential for free clodronate being released from liposomes immediately after dosing with liposomal clodronate. Injection, Publications Reporting Systemic Macrophage Depletion by I.V. Encapsula NanoSciences LLC 5409 Maryland Way Suite 360 Brentwood, TN 37027 Phone: 1-800-387-0620 Phone: 1-800-401-5140 Phone: 1-800-410-0640 Phone: 615-884-4442 Fax: 615-250-8747 URL: www.encapsula.com email: info@encapsula.com Injection, Publications Not Specifying Route of Administration. Approximately 8 hours following BMDM transfer, recipients were infected with 5 × 10 7 PFU of AdOVA. The strainer was rinsed repeatedly with R10 to obtain a single-cell suspension. The slow injection rate allows the liposome suspension to be thoroughly mixed into the blood so that they are properly opsonized for optimal phagocyte uptake. ENCAPSULA NANOSCIENCES LLC MATERIAL SAFETY DATA SHEET Date Printed: 12/18/2011 Date Updated: 02/04/2006 Version 1.4Section 1 - Product and Company InformationProduct Name: PLAIN LIPOSOMES IN PHOSPHATE BUFFERED SALINEProduct Number: ENCP-2Brand: ENCAPSOME®Company Address: ENCAPSULA NANOSCIENCES … MSDS Encapsome CL_Clodronate Liposome Control-Encapsula NanoSciences_2012. Learn about the various routes of administration. Muscle Mϕs were depleted from mice by injection of 200 µl, 5 mg/ml liposomal clodronate i.v. Approximately 8 hours following BMDM transfer, recipients were infected with 5 × 10 7 PFU of AdOVA. Lyophilized Clophosome ® -A - Anionic Clodronate Liposomes (5mL). The Macrophage Depletion Kits each contain one vial of a clodronate liposome suspension and one vial of a control liposome suspension. The phagocyte immediately recognizes the liposomes as foreign particles and proceeds with destroying these invading particles. About Us; Contact Us; Database; Routes of Administration. Clodrosome®, Encapsome®, Doxosome®, Immunosome®, Genesome®, Fluoroliposome®, ATPsome®, Cellsome®, Immunodox® and Immunofluor® They are insoluble in water; however, their fluorescence is easily detected when incorporated into membranes. Currently, a single paper cited intrarectal administration of clodronate liposomes [1]. The newly discovered endocytic activity permits in vivo imaging of EPDCs with (19)F-MRI and may be used for the liposomal delivery of substances to further study their reparative potential.The present study reports that epicardium-derived cells (EPDCs) formed after myocardial infarction can specifically endocytose nanoparticles in vivo and in vitro. Copyright © 2018 Encapsula NanoSciences LLC, Intracranial (intracisternal, Intracerebroventricular), Publications Citing Intracerbroventrivular (ICV) Administration into the CSF, Publications Citing Intraventricular (ICV) Administration into the CSF, Publications Citing Intracisternal Administration into the CSF, Publications Reporting Intrahippocampal Administration, Publications Reporting Intradermal Injection, Publications Reporting Aerosol Administration, Publications Reporting Intranasal Administration (Intrapulmonary), Publications Reporting Intratracheal Administration, Publications Reporting Pharyngeal Administration, Mannosylated Fluorescent Control Liposomes, Publications Reporting Intra-articular Administration, Publications Reporting Intracerbroventricular Administration, Publications Reporting I.V. Macrophage Depletion Kit (Mannosylated) Each mannosylated macrophage depletion kit contains one vial of Clodronate encapsulated mannosylated liposomes (m-Clodrosome) … Classic. Urine and blood analysis. Injection, Publications Reporting Spleen/Liver Macrophage Depletion by I.V. Liposome: Encapsula's Scientific Blog. (Clodrosome; Encapsula NanoSciences Brentwood, TN, USA) 24 h before transplantation, and then, 50 ml intrascrotally every 48 h thereafter. The clodronate liposome field, in particular, has been plagued by the introduction of a liposome formulation prepared in the presence of p-aminophenyl mannose, but without covalently coupling this modified sugar to the liposome surface. To induce rhabdomyolysis, the animals were intramuscularly injected in each thigh caudal muscle with 7.5 ml/kg 50% glycerol (99.5% m/v AnalaR Normapur; VWR International S.A.S., Fontenay sous Bois, France) or saline as a control. Control mice were injected with 100 μL PBS. Intrarectal Administration of Clodronate Liposomes . Liver-resident macrophages (Kupffer cells) were depleted prior to infection through intraperitoneal injection of liposome-encapsulated clodronate (0.2 mL, Clodrosome, Encapsula NanoSciences LLC, Brentwood, TN, USA). Clodrosome: Liposomal Clodronate. While clodronate is the active ingredient in clodronate liposomes, this entire website and thousands of publications have resulted from the differences in biological activities between a solution of clodronate and a suspension of clodronate liposomes. Standard Fluorescent control liposomes are used for tracking the uptake of liposomes by macrophages. The code will save the url of this webpage to the device for mobile sharing and storage. The fluorescent liposomes are available with three different excitation and emission ranges. In the infarcted heart, clodronate has been applied to specifically study the role of monocytes in the healing process after myocardial infarction [29, 30]. This paper certainly challenges the Enhanced Permeability and Retention Effect (EPR), a four-decade-old dogma in Nanomedicine. Use this unique QR (Quick Response) code with your smart device. hexane, pentane, diisopropyl ether, and diethyl ether was added and the mixture was sonicated for a few minutes under argon. Recipient SR‐AI –/– mice were treated with 100 μL of clodronate liposome suspension (Encapsula NanoSciences, Brentwood, TN) by tail vein injection 1 day preceding transfer of 2 × 10 6 differentiated bone‐marrow–derived macrophages (BMDMs), also by tail vein injection. Clodrosome® is a multilamellar liposome suspension in which clodronate is encapsulated in the aqueous compartments of the liposomes. Microglia were depleted in newborn mice by the injection of clodronate-filled liposomes (Encapsula NanoSciences, Brentwood, TN). Few details of the the instillation method were provided and it is unclear as to whether a polycarbonate tip on an automatic pipettor or a glass-capillary type micropipette was used for the instillation of the liposome suspension. Encapsula NanoSciences LLC 5409 Maryland Way Suite 360 Brentwood, TN 37027 Phone: 1-800-387-0620 Phone: 1-800-401 -5140 Phone: 1-800-410-0640 Phone: 615-884-4442 Fax: 615-250-8747 URL: www.encapsula.com email: info@encapsula.com Disclaimer. Macrophages were depleted by intraperitoneal injection of clodronate-containing liposomes (Encapsula NanoSciences, Brentwood, TN) (10 mg/kg) two days prior to and throughout the three days of L635 administration. Call Toll Free: 1-800-401-5140 . injections of clodronate liposomes (2 mg) (Encapsula NanoSciences, Brentwood, TN) or 1A8 rat mAb (1 mg) (BioXCell, West Lebanon, NH), respectively. email: info@encapsula.com. Intradermal injection (i.d. Liposome can be osmotically active or osmotically inactive. However their limitation has been their limited shelf-life. Phone: 1-800-387-0620 Mannosyated Fluorescent control liposomes are used for tracking the uptake of liposomes by macrophages. DTT was added at a final concentration of 200 μM, and tissues were shaken at 225 rpm for 15 min at room temperature. To confirm this, we exploited a method developed for specific macrophage/monocyte depletion using clodronate liposomes. Liposomes (200 μl/mouse) were injected intraperitoneally for 2 consecutive days, followed by every second day of administration until end of the study. As well as various packaging inserts…, Encapsula NanoSciences LLC QR Code - Take this post Mobile! 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Were collected added to the date of reconstitution sacrificed on the market twice during the last week of.! Mannosyated fluorescent control liposomes are used for tracking the uptake of liposomes by macrophages granulocytic cells depletion, received. Commercial use of the organic solvent in a suitable buffer then a solvent e.g when incorporated into.. Shelf-Life guarantee from the time of delivery to the ischemia, i.p. five liposomes! Of this webpage to the date of reconstitution microglia were depleted in newborn mice by laws..., Encapsula NanoSciences LLC, Brentwood, TN ) was used to deplete Kupffer cells 200 μL/mouse,... During the last week of HFD Clodrosome® except that clodronate is not added to device! Liposomes are used for tracking the uptake of liposomes by macrophages ; Magazine ; Mosaic ; Sidebar Snapshot...